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The study of human serum transferrin

dc.contributor.authorBok-cheng, Lim
dc.date.accessioned2011-03-16T22:32:28Z
dc.date.accessioned2022-10-25T05:43:18Z
dc.date.available2011-03-16T22:32:28Z
dc.date.available2022-10-25T05:43:18Z
dc.date.copyright1977
dc.date.issued1977
dc.description.abstractIn this work a chemical affinity column (DEAE-Sephadex Anion Exchanger) with disodium catechol disulphonate as an iron chelator has been used to separate iron that is tightly bound to transferrin in human serum. The results show that all iron tightly bound to transferrin was eluted with bicarbonate-chloride solution at pH 8, and the loosely bound or free iron was retained in the column. The above procedure has been used previously by other workers who have assumed that total serum iron (including loosely bound iron) is eluted with transferrin, contrary to the finding in the present investigation. To determine the total and tightly bound iron in transferrin, atomic absorption was found to be the most convenient and accurate method. Ferrozine method was slow to equilibrate and was subjected to the interference by serum proteins. The ammonium tartrate hastened colour development but the turbidity in the solutions created problems. The isosbestic point of transferrin at 290 nm reported by some previous workers has been shown to be an artifact caused by light scattering of the ultraviolet cuvette. An analytical method for estimating total iron and tightly-bound transferrin iron has been developed and applied to serum samples, from which distinct sex differences are observed.en_NZ
dc.formatpdfen_NZ
dc.identifier.urihttps://ir.wgtn.ac.nz/handle/123456789/23325
dc.languageen_NZ
dc.language.isoen_NZ
dc.publisherTe Herenga Waka—Victoria University of Wellingtonen_NZ
dc.rights.holderAll rights, except those explicitly waived, are held by the Authoren_NZ
dc.rights.licenseAuthor Retains Copyrighten_NZ
dc.rights.urihttps://www.wgtn.ac.nz/library/about-us/policies-and-strategies/copyright-for-the-researcharchive
dc.subjectIron in the bodyen_NZ
dc.subjectSerumen_NZ
dc.subjectTransferrinen_NZ
dc.titleThe study of human serum transferrinen_NZ
dc.typeTexten_NZ
thesis.degree.disciplineChemistryen_NZ
thesis.degree.grantorTe Herenga Waka—Victoria University of Wellingtonen_NZ
thesis.degree.levelMastersen_NZ
thesis.degree.nameMaster of Scienceen_NZ
vuwschema.type.vuwAwarded Research Masters Thesisen_NZ

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